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In vivo performance of dual ligand augmented endothelialized expanded polytetrafluoroethylene vascular grafts
Author(s) -
Chan Bernard P.,
Liu Wenge,
Klitzman Bruce,
Reichert William M.,
Truskey George A.
Publication year - 2004
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30114
Subject(s) - polytetrafluoroethylene , in vivo , biomedical engineering , ligand (biochemistry) , dual (grammatical number) , chemistry , materials science , medicine , composite material , biology , receptor , microbiology and biotechnology , art , literature
Abstract In this study, we examined combinations of three approaches to improve the adhesion of endothelial cells (EC) onto expanded polytetrafluoroethylene (ePTFE) vascular grafts placed at the femoral artery of rats: (1) high‐affinity receptor‐ligand binding of RGD–streptavidin (SA) and biotin to supplement integrin‐mediated EC adhesion; (2) cell sodding to pressurize the seeded EC into the interstices of the ePTFE grafts; and (3) longer postseeding attachment time from 1 to 24 h prior to implantation. An in vitro system, which accounts for cell loss due to both graft handling and shear stress, was designed to optimize conditions for in vivo experiments. Results suggest that longer in vitro attachment time enabled the adherent EC to endure mechanical stresses by forming strong adhesions to the underlying extracellular matrix substrates; cell sodding helped to retain the adherent EC by physically docking the cells against the graft interstices; and the SA–biotin interaction enhanced the early attachment of EC but did not lead to better cell retention or reduced surface coverage of blood clot in the current study. Mechanical manipulation of cells during implantation is a limiting factor in maintaining a confluent EC layer on synthetic vascular grafts. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 72B: 52–63, 2005