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Vascular graft healing. III. FTIR analysis of ePTFE graft samples from implanted bigrafts
Author(s) -
MurrayWijelath Jacqueline,
Lyman Donald J.,
Wijelath Errol S.
Publication year - 2004
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30044
Subject(s) - fibrin , wound healing , chemistry , polytetrafluoroethylene , fourier transform infrared spectroscopy , biomedical engineering , surgery , medicine , immunology , chemical engineering , engineering , organic chemistry
A bigraft, composed of a 30‐μm internodal distance expanded polytetrafluoroethylene (ePTFE) arterial graft segment and a preclotted polyethylene terephthalate arterial graft segment, was used to study the healing process of two different materials in the same dog. Healing was followed by FTIR‐ATR spectroscopy and correlated with ELISA analyses of selected growth factors and matrix proteins. The FTIR analyses of the ePTFE explants from 1 and 3 h; 3, 5, 7, 10, and 14 days; and 4 and 8 weeks showed that the ePTFE grafts did not heal (endothelialize), but appeared to remain in overlapping inflammation and proliferation stages of wound healing. Although FN was found in the fibrin layer coating the luminal surface of the ePTFE, VEGF was not present. The inability of VEGF to complex with FN may be the result of FN binding sites being blocked or hidden by the conformation of the fibrin clot that forms on ePTFE. Also, TGF‐β was not present beyond the initial clot formation at 3 h postimplantation. The absence of these two growth factors may be an important factor in the lack of healing of 30‐μm ePTFE grafts, because both were found to be present during the early stages of healing for the preclotted polyester grafts that did endothelialize. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 70B: 223–232, 2004