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In vitro cytotoxicity testing of AB‐polymer networks based on oligo(ϵ‐caprolactone) segments after different sterilization techniques
Author(s) -
Rickert Dorothee,
Lendlein A.,
Schmidt A. M.,
Kelch S.,
Roehlke W.,
Fuhrmann R.,
Franke R. P.
Publication year - 2003
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.10069
Subject(s) - caprolactone , sterilization (economics) , in vitro , cytotoxicity , chemistry , polymer , organic chemistry , polymerization , biochemistry , business , foreign exchange market , finance , exchange rate
Recently developed versatile biodegradable polymeric biomaterial offer new therapeutic options in numerous medical fields. Biocompatibility is a crucial requirement for the biomedical application of biomaterials, including the sterilization of these materials with the use of accepted protocols. Ethylene‐oxide (EO) and low‐temperature plasma (LTP) sterilization are frequently used low‐temperature sterilization technologies for heat‐sensitive materials. The agarose diffusion assay is a recommended cell‐screening test to assess the cytotoxicity of biomaterials in vitro . The sensitivity of the agarose assay can be increased by using a modified computer‐based image‐analysis system. The influence of EO and LTP sterilization on the cytotoxicity of a versatile polymer system of shape‐memory polymer networks based on oligo (ϵ‐caprolactone) dimethacrylate and n‐butyl acrylate was investigated. Statistically significant differences in the rate of cell lysis after EO and LTP sterilization of the polymer samples were detected by using this modified quantification system. The influence of the different sterilization techniques on the cytotoxicity of the polymeric material, as well as the clinical relevance of the described differences, are discussed. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 67B: 722–731, 2003