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Osteoconductive and hemostatic properties of apatite formed on/in agarose gel as a bone‐grafting material
Author(s) -
Tabata Masashi,
Shimoda Toru,
Sugihara Kazumasa,
Ogomi Daisuke,
Serizawa Takeshi,
Akashi Mitsuru
Publication year - 2003
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.10063
Subject(s) - agarose , hemostasis , biomedical engineering , chemistry , materials science , grafting , dentistry , surgery , chromatography , composite material , medicine , polymer
The biologic behavior of hydroxyapatite formed on/in agarose (HA/agarose) gels with the use of a novel alternate soaking process was compared with commercially available Bone Ject (True‐Bone ceramic–collagen combined bone‐graft material, Koken, Japan) as a filler for the tooth‐extraction sockets of six adult monkeys ( Macasa fascicularis ). After the monkeys' first premolars were extracted, the defects created were replaced with one of the following materials: (a) HA/agarose created by 12 soaking cycles, (b) HA/agarose created by 9 soaking cycles, (c) Bone Ject, and (d) no material implantation (control). The time of hemostasis in each extraction site was estimated, and the samples were then studied histologically. In the controls, the time of hemostasis was about 5 min. The Bone Ject particles were easily washed out by bleeding, and the time of hemostasis was about 15 min. The HA/agarose gel was densely packed into the bony defect. The hemorrhage from the defects stopped within a few seconds after graft placement. This hemostasis was most likely related to the compactibility and adhesiveness of the material. After 12 weeks of implantation, free Bone Ject particles surrounded by inflammatory cells were observed. The bony defect filled with the HA/agarose gels was completely absorbed and replaced by newly formed bone possessing bone marrow. There was no difference in the biologic behavior of HA/agarose gels created by 9 versus 12 soaking cycles. The present study suggests that HA/agarose gels may play an important role as an alternative biodegradable bone‐graft material for autogenous bone in humans. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 67B: 680–688, 2003