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Host protection against deliberate bacterial contamination of an extracellular matrix bioscaffold versus Dacron™ mesh in a dog model of orthopedic soft tissue repair
Author(s) -
Badylak Stephen F.,
Ching Wu Ching,
Bible Melissa,
McPherson Edward
Publication year - 2003
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.10062
Subject(s) - extracellular matrix , implant , scaffold , soft tissue , orthopedic surgery , regeneration (biology) , surgery , extracellular , medicine , chemistry , biomedical engineering , biology , microbiology and biotechnology , biochemistry
Abstract The resistance of two biomaterials, one synthetic and one biologic in origin, to deliberate bacterial infection was compared in a dog model of orthopedic soft tissue reconstruction. Twenty‐four adult female dogs were randomly divided into two equal groups and a 2.0‐cm‐round full‐thickness defect was created on the lateral surface of the stifle joint, leaving only the synovium and skin intact. The defect was surgically repaired with either Dacron™ mesh or a porcine derived extracellular matrix (ECM) scaffold material. The repair site was inoculated with 1 × 10 8 Staphylococcus aureus at the time of surgery and the dogs were survived for 28 days. Results showed a chronic pyogranulomatous inflammatory response at the Dacron™ implant sites versus a constructive tissue‐remodeling response without residual inflammation at the ECM implant site. Three dogs in the group receiving the Dacron™ mesh were treated with Keflex™ (500 mg bid × 7 days) for signs of septicemia. A quantitative bacterial count of the implant sites at the time of sacrifice showed 6.52 × 10 5 ± 1.2 × 10 6 and 6.5 × 10 2 ± 1.8 × 10 3 bacteria per gram of tissue for the Dacron™ and ECM scaffold sites, respectively ( P < .03). The ECM implant material was more resistant than the synthetic implant material to persistent infection following deliberate bacterial contamination and the ECM scaffold supported constructive tissue remodeling. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 67B: 648–654, 2003