Premium
Sustained released of bioactive mesenchymal stromal cell‐derived extracellular vesicles from 3D‐printed gelatin methacrylate hydrogels
Author(s) -
Born Louis J.,
McLoughlin Shan T.,
Dutta Dipankar,
Mahadik Bhushan,
Jia Xiaofeng,
Fisher John P.,
Jay Steven M.
Publication year - 2022
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.37362
Subject(s) - mesenchymal stem cell , self healing hydrogels , gelatin , materials science , stromal cell , methacrylate , biomedical engineering , in vivo , extracellular vesicles , biophysics , nanotechnology , microbiology and biotechnology , chemistry , biochemistry , copolymer , polymer chemistry , biology , composite material , cancer research , medicine , polymer
Abstract Extracellular vesicles (EVs) represent an emerging class of therapeutics with significant potential and broad applicability. However, a general limitation is their rapid clearance after administration. Thus, methods to enable sustained EV release are of great potential value. Here, we demonstrate that EVs from mesenchymal stem/stromal cells (MSCs) can be incorporated into 3D‐printed gelatin methacrylate (GelMA) hydrogel bioink, and that the initial burst release of EVs can be reduced by increasing the concentration of crosslinker during gelation. Further, the data show that MSC EV bioactivity in an endothelial gap closure assay is retained after the 3D printing and photocrosslinking processes. Our group previously showed that MSC EV bioactivity in this assay correlates with pro‐angiogenic bioactivity in vivo, thus these results indicate the therapeutic potential of MSC EV‐laden GelMA bioinks.