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Preparation of a novel bovine cancellous bone/poly‐amino acid composite with low immunogenicity, proper strength, and cytocompatibility in vitro
Author(s) -
Luo Lin,
Li Shuyang,
Ji Mizhi,
Ding Zhengwen,
Yan Yonggang,
Yin Jie,
Xiong Yi
Publication year - 2021
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.37139
Subject(s) - materials science , composite number , immunogenicity , cancellous bone , mesenchymal stem cell , extrusion , in vitro , immune system , composite material , biomedical engineering , chemical engineering , biochemistry , chemistry , immunology , biology , medicine , anatomy , engineering , microbiology and biotechnology
In this work, the delipidized and deproteinized bovine cancellous bone powder/poly–amino acid (DDBP/PAA) composite was fabricated by extrusion‐injection molding method for the first time. After about 70% clearance rate by the delipidization and deproteinization procedures, the residual antigens of galactosyl α–(1, 3)–galactosyl β–1,4–N–aeetylglueosaminyl (α–Gal) and major histocompatibility complex (MHC) II were basically eliminated by the extrusion–injection molding process, which may cause high titer of antibody and lead to hyperacute rejection or chronic immune toxicity. Meanwhile, the natural BMP II and apatite in bovine bone were kept in DDBP/PAA composite. After 26 weeks of immersion in simulated body fluid, the DDBP/PAA composite remained the intact appearance, 96.4% of weight, and 69.2% of compressive strength, and these showed sufficient degradation stability. The composite also exhibited excellent attachment and proliferation abilities of mouse bone marrow mesenchymal stem cells (mMSCs). The results herein suggested that the DDBP/PAA composite was expected to be a load–bearing transplant with some natural ingredients for hard tissue repair.