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Effect of manganese, zinc, and copper on the biological and osteogenic properties of mesoporous bioactive glass nanoparticles
Author(s) -
Westhauser Fabian,
Wilkesmann Sebastian,
Nawaz Qaisar,
Hohenbild Frederike,
Rehder Felix,
Saur Merve,
Fellenberg Jörg,
Moghaddam Arash,
Ali Muhammad S.,
Peukert Wolfgang,
Boccaccini Aldo R.
Publication year - 2021
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.37136
Subject(s) - materials science , zinc , bioactive glass , viability assay , mesoporous material , alkaline phosphatase , nanoparticle , manganese , copper , stromal cell , extracellular matrix , chemical engineering , nuclear chemistry , nanotechnology , cell , biochemistry , enzyme , metallurgy , chemistry , cancer research , composite material , biology , engineering , catalysis
Mesoporous bioactive glass nanoparticles (MBGNs) have demonstrated promising properties for the local delivery of therapeutically active ions with the aim to improve their osteogenic properties. Manganese (Mn), zinc (Zn), and copper (Cu) ions have already shown promising pro‐osteogenic properties. Therefore, the concentration‐dependent impact of MBGNs (composition in mol%: 70 SiO 2 , 30 CaO) and MBGNs containing 5 mol% of either Mn, Zn, or Cu (composition in mol%: 70 SiO 2 , 25 CaO, 5 MnO/ZnO/CuO) on the viability and osteogenic differentiation of human marrow‐derived mesenchymal stromal cells (BMSCs) was assessed in this study. Mn‐doped MBGNs (5Mn‐MBGNs) showed a small “therapeutic window” with a dose‐dependent negative impact on cell viability but increasing pro‐osteogenic features alongside increasing Mn concentrations. Due to a constant release of Zn, 5Zn‐MBGNs showed good cytocompatibility and upregulated the expression of genes encoding for relevant members of the osseous extracellular matrix during the later stages of cultivation. In contrast to all other groups, BMSC viability increased with increasing concentration of Cu‐doped MBGNs (5Cu‐MBGNs). Furthermore, 5Cu‐MBGNs induced an increase in alkaline phosphatase activity. In conclusion, doping with Mn, Zn, or Cu can enhance the biological properties of MBGNs in different ways for their potential use in bone regeneration approaches.