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Spatiotemporal histological changes observed in mouse subcutaneous tissues during the foreign body reaction to silicone
Author(s) -
Oe Sao,
Masum Md. Abdul,
Ichii Osamu,
Nishimura Takanori,
Nakamura Teppei,
Namba Takashi,
Otani Yuki,
Nakayama Yasuhide,
Elewa Yaser Hosny Ali,
Kon Yasuhiro
Publication year - 2021
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.37115
Subject(s) - connective tissue , inflammation , subcutaneous tissue , foreign body , reticular connective tissue , silicone , materials science , foreign body giant cell , pathology , mast cell , subcutaneous injection , medicine , immunology , endocrinology , composite material , surgery
We investigated spatiotemporal changes in host tissues during foreign body reactions. Silicone tube was subcutaneously embedded into ICR mice, and tissue surrounding silicone (TSS) was observed at 2, 7, 14, 21, 28, 43, and 70 days (D) postsurgery. The thin layer (TL) and loose connective tissues (LCTs) (inside and outside the TSS) developed until D21 and densified afterward. Neutrophils infiltrated the TSS until D14 and formed neutrophil extracellular traps (NETs) in the TL during D7‐21. In the LCTs, mast cell counts increased until D21, and macrophage numbers peaked at D14. Several macrophages showed LYVE‐1 expression, supporting a tissue‐remodeling role. Developmental indices of collagen fibers (CFs) and reticular fibers (RFs) increased during D2‐21. NETs, but not neutrophils, were detected after D28. Mast cell numbers peaked at D43 and were maintained until D70. Myofibroblasts consistently localized to the TL from D14. During D21‐28, the area of connective tissue (CNT), and CFs and RFs decreased and increased, respectively, and both remained constant during D28‐70. The CF density remained constant from D21 and increased at D70. Thus, TSS showed two phases: inflammation and CNT development (D2‐21), and inflammation convergence and CNT stabilization (D28‐70). These results provide insights into foreign body reactions in clinical cases.