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The performance of 3D bioscaffolding based on a human periodontal ligament stem cell printing technique
Author(s) -
Tian Yinping,
Liu Minyi,
Liu Yaoyao,
Shi Changzheng,
Wang Yayu,
Liu Tong,
Huang Yi,
Zhong Peihua,
Dai Jian,
Liu Xiangning
Publication year - 2021
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.37114
Subject(s) - materials science , periodontal ligament stem cells , biocompatibility , gelatin , 3d bioprinting , self healing hydrogels , biomedical engineering , tissue engineering , alkaline phosphatase , chemistry , polymer chemistry , medicine , biochemistry , metallurgy , enzyme
Abstract Bone tissue plays an important role in supporting and protecting the structure and function of the human body. Bone defects are a common source of injury and there are many reconstruction challenges in clinical practice. However, 3D bioprinting of scaffolds provides a promising solution. Hydrogels have emerged as biomaterials with good biocompatibility and are now widely used as cell‐loaded materials for bioprinting. This study involved three steps: First, sodium alginate (SA), gelatin (Gel), and nano‐hydroxyapatite (na‐HA) were mixed into a hydrogel and its rheological properties assessed to identify the optimum slurry for printing. Second, SA/Gel/na‐HA bioscaffolds were printed using 3D bioprinting technology and their physical properties characterized for surface morphology, swelling, and mechanical properties. Finally, human periodontal ligament stem cells (hPDLSCs) were mixed with SA/Gel/na‐HA printing slurry to create a “bioink” to prepare SA/Gel/na‐HA/ hPDLSCs cell bioscaffolds. These were tested for biocompatibility and osteogenic differentiation performance using live/dead cell staining, cell adhesion, cell proliferation, and alkaline phosphatase activity. The SA/Gel/na‐HA hydrogel exhibited shear‐thinning behavior. The equilibrium swelling of the bioscaffold was 125.9%, the compression stress was 0.671 MPa, and the compression elastic modulus was 8.27 MPa. The SA/Gel/na‐HA/hPDLSCs cell bioscaffolds caused effective stimulation of cell survival, proliferation, and osteoblast differentiation. Therefore, the SA/Gel/na‐HA/hPDLSCs cell bioscaffolds displayed potential as a material for bone defect reconstruction.