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Low‐intensity pulsed ultrasound promotes the formation of periodontal ligament stem cell sheets and ectopic periodontal tissue regeneration
Author(s) -
Li Han,
Zhou Jie,
Zhu Mengyuan,
Ying Siqi,
Li Lingjie,
Chen Duanjing,
Li Jie,
Song Jinlin
Publication year - 2021
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.37102
Subject(s) - periodontal ligament stem cells , periodontal fiber , alizarin red , low intensity pulsed ultrasound , tissue engineering , alkaline phosphatase , staining , transplantation , stem cell , extracellular matrix , regeneration (biology) , dental follicle , materials science , biomedical engineering , chemistry , microbiology and biotechnology , dentistry , pathology , biology , medicine , ultrasound , therapeutic ultrasound , surgery , biochemistry , radiology , enzyme
Human periodontal ligament stem cells (hPDLSCs) sheets play an important role in periodontal tissue engineering. Low‐intensity pulsed ultrasound (LIPUS) has been reported as an effective stimulus to regulate cell biological behavior. The present study aims to explore the potential of LIPUS to promote the formation and function of hPDLSC sheets (hPDLSCSs). Hematoxylin–eosin (H&E) staining, western blot, real‐time PCR, alkaline phosphatase (ALP), and alizarin red staining were used to evaluate the formation and osteogenic effect of LIPUS on hPDLSCSs in vitro . Hydroxyapatite with or without hPDLSCSs was transplanted in the subcutaneous pockets on the back of nude mice and histological analysis was performed. H&E staining showed increased synthesis of extracellular matrix (ECM) and real‐time PCR detected a significant increase in ECM‐related genes after LIPUS treatment. In addition, LIPUS could promote the expression of osteogenic differentiation‐related genes and proteins. ALP and alizarin red staining also found LIPUS enhanced the osteogenesis of hPDLSCSs. After transplantation in vivo , more dense collagen fibers similar to periodontal ligament were regenerated. Collectively, these results indicate that LIPUS not only promotes the formation and osteogenic differentiation of hPDLSCSs but also is a potential treatment strategy for periodontal tissue engineering.