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Control of cellular adhesiveness in hyaluronic acid‐based hydrogel through varying degrees of phenol moiety cross‐linking
Author(s) -
Bagheri Sara,
Bagher Zohreh,
Hassanzadeh Sajad,
Simorgh Sara,
Kamrava Seyed Kamran,
Nooshabadi Vajihe Taghdiri,
Shabani Ronak,
Jalessi Maryam,
Khanmohammadi Mehdi
Publication year - 2021
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.37049
Subject(s) - self healing hydrogels , hyaluronic acid , biocompatibility , materials science , adhesive , biomaterial , tissue engineering , adhesion , cell adhesion , moiety , horseradish peroxidase , aqueous solution , phenol , conjugated system , polymer chemistry , biophysics , nanotechnology , chemistry , biomedical engineering , biochemistry , organic chemistry , polymer , composite material , medicine , genetics , layer (electronics) , metallurgy , biology , enzyme
Current hyaluronic acid‐based hydrogels often cause cytotoxicity to encapsulated cells and lack the adhesive property required for effective biomedical and tissue engineering applications. Provision of the cell‐adhesive surface is an important requirement to improve its biocompatibility. An aqueous solution of hyaluronic acid possessing phenolic hydroxyl (HA‐Ph) moieties is gellable via a horseradish peroxidase (HRP)‐catalyzed oxidative cross‐linking reaction. This study evaluates the effect of different degrees of cross‐linked Ph moieties on cellular adhesiveness and proliferation on the resultant enzymatically cross‐linked HA‐Ph hydrogels. Mechanical characterization demonstrated that the compression force of engineered hydrogels could be tuned in the range of 0.05–35 N by changing conjugated Ph moieties in the precursor formulation. The water contact angle and water content show hydrophobicity of hydrogels increased with increasing content of cross‐linked Ph groups. The seeded mouse embryo fibroblast‐like cell line and human cervical cancer cell line, on the HA‐Ph hydrogel, proved cell attachment and spreading with a high content of cross‐linked Ph groups. The HA‐Ph with a higher degree of Ph moieties shows the maximum degree of cell adhesion, spreading, and proliferation which presents this hydrogel as a suitable biomaterial for biomedical and tissue engineering applications.