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Myocardial injection of a thermoresponsive hydrogel with reactive oxygen species scavenger properties improves border zone contractility
Author(s) -
Spaulding Kimberly A.,
Zhu Yang,
Takaba Kiyoaki,
Ramasubramanian Anusuya,
Badathala Anusha,
Haraldsson Henrik,
Collins Alexander,
Aguayo Esteban,
Shah Curran,
Wallace Arthur W.,
Ziats Nicholas P.,
Lovett David H.,
Baker Anthony J.,
Healy Kevin E.,
Ratcliffe Mark B.
Publication year - 2020
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36941
Subject(s) - contractility , reactive oxygen species , myocardial infarction , border zone , materials science , ethylene glycol , oxygen , medicine , cardiology , chemistry , biochemistry , organic chemistry
The decrease in contractility in myocardium adjacent (border zone; BZ) to a myocardial infarction (MI) is correlated with an increase in reactive oxygen species (ROS). We hypothesized that injection of a thermoresponsive hydrogel, with ROS scavenging properties, into the MI would decrease ROS and improve BZ function. Fourteen sheep underwent antero‐apical MI. Seven sheep had a comb‐like copolymer synthesized from N ‐isopropyl acrylamide (NIPAAm) and 1500 MW methoxy poly(ethylene glycol) methacrylate, (NIPAAm‐PEG1500), injected (20 × 0.5 mL) into the MI zone 40 min after MI (MI + NIPAAm‐PEG1500) and seven sheep were MI controls. Cardiac MRI was performed 2 weeks before and 6 weeks after MI + NIPAAm‐PEG1500. BZ wall thickness at end systole was significantly higher for MI + NIPAAm‐PEG1500 (12.32 ± 0.51 mm/m 2 MI + NIPAAm‐PEG1500 vs. 9.88 ± 0.30 MI; p = .023). Demembranated muscle force development for BZ myocardium 6 weeks after MI was significantly higher for MI + NIPAAm‐PEG1500 (67.67 ± 2.61 m N /m 2 MI + NIPAAm‐PEG1500 vs. 40.53 ± 1.04 MI; p < .0001) but not significantly different from remote myocardium or BZ or non‐operated controls. Levels of ROS in BZ tissue were significantly lower in the MI + NIPAAm‐PEG1500 treatment group (hydroxyl p = .0031; superoxide p = .0182). We conclude that infarct injection of the NIPAAm‐PEG1500 hydrogel with ROS scavenging properties decreased ROS and improved contractile protein function in the border zone 6 weeks after MI.