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A novel pathway to produce biodegradable and bioactive PLGA/TiO 2 nanocomposite scaffolds for tissue engineering: Air–liquid foaming
Author(s) -
Pelaseyed Seyedeh S.,
Madaah Hosseini Hamid R.,
Samadikuchaksaraei Ali
Publication year - 2020
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36910
Subject(s) - materials science , simulated body fluid , plga , biocompatibility , nanocomposite , apatite , chemical engineering , porosity , polymer , solvent , tissue engineering , biodegradable polymer , compressive strength , composite material , scanning electron microscope , biomedical engineering , nanoparticle , nanotechnology , organic chemistry , chemistry , medicine , engineering , metallurgy
Poly (lactate‐co‐glycolate) (PLGA) is a typical biocompatible and biodegradable synthetic polymer. The addition of TiO 2 nanoparticles has shown to improve compressive modulus of PLGA scaffolds and reduced fast degradation. A novel method has been applied to fabricate PLGA/TiO 2 scaffolds without using any inorganic solvent, with aim of improving the biocompatibility, macroscale morphology, and well inter‐connected pores efficacy: Air–Liquid Foaming. Field Emission Scanning Electron Microscopy (FESEM) revealed an increase in interconnected porosity of up to 98%. As well the compressive testing showed enhancement in modulus. Bioactivity and in vitro degradation were studied with immersion of scaffolds in Simulated Body Fluid (SBF) and incubation in Phosphate Buffered Saline (PBS), respectively. Formation of apatite layer corroborated the bioactivity after soaking in SBF. Degradation rate of scaffolds was increased with excessive addition of TiO 2 contents withal. The in vitro cultured human‐like MG63 ostoblast cells showed attachment, proliferation, and nontoxcitiy in contact, using MTT assay [3‐(4, 5‐Dimethylthiazol‐2‐yl)‐2, 5‐Diphenyltetrazolium Bromide]. According to the results, the novel method utilized in this study generated porous viable tissue without using any inorganic solvent or porogen can be a promising candidate in further treatment of orthopedic patients effectively.