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Effect of scaffold properties on adhesion and maintenance of boundary cap neural crest stem cells in vitro
Author(s) -
Han Yilin,
Baltriukienė Daiva,
Kozlova Ele.
Publication year - 2020
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36900
Subject(s) - materials science , stem cell , scaffold , microbiology and biotechnology , tissue engineering , neural crest , biomedical engineering , adult stem cell , population , in vitro , biophysics , biology , endothelial stem cell , biochemistry , engineering , embryo , demography , sociology
Optimal combination of stem cells and biocompatible support material is a promising strategy for successful tissue engineering. The required differentiation of stem cells is crucial for functionality of engineered tissues and can be regulated by chemical and physical cues. Here we examined how boundary cap neural crest stem cells (bNCSCs) are affected when cultured in the same medium, but on collagen‐ or laminin‐polyacrylamide (PAA) scaffolds of different stiffness (0.5, 1, or ~7 kPa). bNCSCs displayed marked differences in their ability to attach, maintain a large cell population and differentiate, depending on scaffold stiffness. These findings show that the design of physical cues is an important parameter to achieve optimal stem cell properties for tissue repair and engineering.