Premium
Nail matrix regenerative engineering: in vitro evaluation of poly(lactide‐ co ‐glycolide)/gelatin fibrous substrates
Author(s) -
Montgomery Aundrya B.,
McClinton Aneesah,
Nair Lakshmi,
Laurencin Cato T.
Publication year - 2020
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36888
Subject(s) - materials science , gelatin , nail matrix , biomedical engineering , plga , nail (fastener) , tissue engineering , soft tissue , dentistry , nail plate , surgery , medicine , nanotechnology , chemistry , biochemistry , nanoparticle , metallurgy , complication
Acute traumatic nail injury treatment repair procedures are commonly conducted in emergency departments and primary care offices. Current repair methods use nail splints that are inserted within the nail root to prevent the fusion of the proximal nail fold and the matrix tissue. Splints provide a protective barrier overlying the nail bed soft tissue during recovery periods, but uncertain prognoses (i.e., aesthetic and functional disadvantages) reveal a need for improved nail repair techniques. Nail splints are not specifically designed for nail organ restoration via biological mechanisms, thus, a clinical application that utilizes regenerative engineering techniques can prove useful in improving the nail injury prognoses. Using the coaxial electrospinning method, hybrid poly(lactide‐ co ‐glycolide) (PLGA) (85:15) and gelatin fibrous scaffolds (Hybrid 1: PLGA shell, gelatin core and Hybrid 2 : gelatin shell, PLGA core) with average fiber diameters of 540 ± 118 and 2,215 ± 1,135 nm, respectively, were produced and successful encapsulation of core fibers was observed. Furthermore, nail stem cells expressing stem cell characteristic markers CD90, CD29, and Lgr6 showed preferred attachment to Hybrid 2 scaffolds after 24 hr. Overall, an in vitro regenerative engineered nail matrix may aid to improve the cosmetic appearance and function of injured nail organs post‐traumatic injury.