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Fabrication of porous carbonate apatite granules using microfiber and its histological evaluations in rabbit calvarial bone defects
Author(s) -
Akita Kazuya,
Fukuda Naoyuki,
Kamada Kumiko,
Kudoh Keiko,
Kurio Naito,
Tsuru Kanji,
Ishikawa Kunio,
Miyamoto Youji
Publication year - 2020
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36850
Subject(s) - microfiber , materials science , porosity , apatite , dissolution , electrospinning , biomedical engineering , carbonate , chemical engineering , composite material , polymer , metallurgy , medicine , engineering
Carbonate apatite (CO 3 Ap) granules are known to show good osteoconductivity and replaced to new bone. On the other hand, it is well known that a porous structure allows bone tissue to penetrate its pores, and the optimal pore size for bone ingrowth is dependent on the composition and structure of the scaffold material. Therefore, the aim of this study was to fabricate various porous CO 3 Ap granules through a two‐step dissolution–precipitation reaction using CaSO 4 as a precursor and 30‐, 50‐, 120‐, and 205‐μm diameter microfibers as porogen and to find the optimal pore size of CO 3 Ap. Porous CO 3 Ap granules were successfully fabricated with pore size 8.2–18.7% smaller than the size of the original fiber porogen. Two weeks after the reconstruction of rabbit calvarial bone defects using porous CO 3 Ap granules, the largest amount of mature bone was seen to be formed inside the pores of CO 3 Ap (120) [porous CO 3 Ap granules made using 120‐μm microfiber] followed by CO 3 Ap (50) and CO 3 Ap (30). At 4 and 8 weeks, no statistically significant difference was observed based on the pore size, even though largest amount of mature bone was formed in case of CO 3 Ap (120). It is concluded, therefore, that the optimal pore size of the CO 3 Ap is that of CO 3 Ap (120), which is 85 μm.