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The characteristic regulation of gene expression Lbp and Sod3 in peri‐implant connective tissue of rats
Author(s) -
Kobayashi Takafumi,
Sasaki Hodaka,
Asami Yosuke,
Mori Gentaro,
Yoshinari Masao,
Yajima Yasutomo
Publication year - 2020
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36839
Subject(s) - connective tissue , downregulation and upregulation , gene expression , pathology , superoxide dismutase , microarray analysis techniques , biology , immunohistochemistry , microbiology and biotechnology , gene , medicine , endocrinology , genetics , oxidative stress
The aim of this study was to investigate the characteristic gene expression profile and localization of peri‐implant connective tissue (PICT) compared with those of periodontal connective tissue (PCT) and oral mucosal connective tissue (OMCT). Upper first molar of 5‐week‐old rats were extracted and titanium implant were placed for PICT group. PCT and OMCT were used as control. Laser microdissected connective tissue at 4 weeks used for microarray analysis. The expression and localization of selected genes were confirmed by quantitative real‐time PCR (qRT‐PCR) and immunohistochemistry. Approximately, 1000 genes of upregulated and downregulated in PICT compared with PCT and OMCT were recognized. Based on the results of microarray analysis and qRT‐PCR were demonstrated lipopolysaccharide binding protein ( Lbp ) as a specific upregulated gene and superoxide dismutase 3 ( Sod3 ) as a specific downregulated gene in PICT. Immunoreaction of LBP and F4/80 as macrophage marker localized to subepithelial and implant facing connective tissue in PICT. SOD3 expression was not observed in PICT, reactive oxygen species, a target of superoxide dismutase, was strongly and locally expressed in all three tissues. Our data suggested that the upregulation of Lbp and downregulation of Sod3 are as characteristic gene expression pattern in PICT.