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Immobilization of BMP‐2, BMP‐7 and alendronic acid on titanium surfaces: Adhesion, proliferation and differentiation of bone marrow‐derived stem cells
Author(s) -
Kämmerer Peer W.,
Pabst Andreas M.,
Dau Michael,
Staedt Henning,
AlNawas Bilal,
Heller Martin
Publication year - 2020
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36805
Subject(s) - bone morphogenetic protein 2 , alkaline phosphatase , osteocalcin , cell growth , titanium , materials science , cell adhesion , adhesion , stem cell , bone marrow stem cell , cell , microbiology and biotechnology , andrology , in vitro , chemistry , biochemistry , biology , medicine , metallurgy , enzyme , composite material
This study analyzed the influence of titanium (TiO 2 ) surface modifications with two osteogenic proteins (BMP‐2, BMP‐7) and an anti‐osteoclastic drug (alendronic acid [AA]) on sandblasted/acid‐etched (SLA) and plain TiO 2 (PT) on cell adhesion, proliferation and differentiation (alkaline phosphatase [AP] and osteocalcin [OC]) of bone‐marrow derived stem cells (BMSCs) after 1, 3 and 7 days in‐vitro. Initially, AA surfaces showed the highest cell number and surface coverage. At day 3 and 7, BMP and AA‐modified surfaces exhibited a significantly enhanced cell growth. For proliferation, at days 3 and 7, an enhancement on BMP‐2, BMP‐7 and AA‐surfaces was seen. At day 7, SLA also showed a higher proliferation when compared to PT. Initially, AP expression was elevated on SLA and AA surfaces. At days 3 and 7, a significant increased AP expression was seen for SLA, BMP‐2, BMP‐7 and AA discs. For OC, SLA and AA surfaces had the highest expression after 1 day whereas after 3 and 7 days a significant difference was recorded for SLA, BMP‐2, BMP‐7 and AA. In conclusion, a beneficial biological effect of a chemical immobilization method of BMP‐2, BMP‐7 and alendronate onto titanium surfaces on BMSCs was proven.