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Sterilized chitosan‐based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity
Author(s) -
RodríguezRodríguez Rogelio,
VelasquilloMartínez Cristina,
Knauth Peter,
López Zaira,
MorenoValtierra Maira,
BravoMadrigal Jorge,
JiménezPalomar Inés,
LunaBárcenas Gabriel,
EspinosaAndrews Hugo,
GarcíaCarvajal Zaira Y.
Publication year - 2020
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36794
Subject(s) - self healing hydrogels , materials science , biocompatibility , polyvinyl alcohol , chitosan , crystallinity , chemical engineering , fourier transform infrared spectroscopy , thermal stability , nuclear chemistry , mtt assay , polymer , polymer chemistry , composite material , chemistry , in vitro , biochemistry , engineering , metallurgy
Gelatin/chitosan/polyvinyl alcohol hydrogels were fabricated at different polymer ratios using the freeze‐drying and sterilized by steam sterilization. The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X‐ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3‐4,[5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively. The samples showed a decomposition onset temperature below 253.3 ± 4.8°C, a semicrystalline nature, and a highly porous structure. Hydrogels reached the maximum water uptake in phosphate‐buffered saline after 80 min, showing values from nine to twelve times their dry mass. Also, hydrogels exhibiting a solid‐like behavior ranging from 2,567 ± 467 to 48,705 ± 2,453 Pa at 0.1 rad/s (low frequency). The sterilized hydrogels showed low cytotoxicity (cell viability > 70%) to the HT29‐MTX‐E12 cell line. Sterilized hydrogels by steam sterilization can be good candidates as scaffolds for tissue engineering applications.