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Impact of crystallinity and crystal size of nanostructured carbonated hydroxyapatite on pre‐osteoblast in vitro biocompatibility
Author(s) -
dos Anjos Suzana,
Mavropoulos Elena,
Alves Gutemberg G.,
Costa Andrea M.,
de Alencar Hausen Moema,
Spiegel Caroli.,
Longuinho Mariana M.,
Mir Mirta,
Granjeiro José M.,
Rossi Alexandre M.
Publication year - 2019
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36709
Subject(s) - crystallinity , biocompatibility , materials science , osteoblast , biomaterial , centrifugation , nanoparticle , in vivo , in vitro , transmission electron microscopy , biophysics , protein adsorption , nanotechnology , chemistry , biochemistry , polymer , composite material , biology , metallurgy , microbiology and biotechnology
Nanostructured carbonated hydroxyapatite (nCHA) is a promising biomaterial for bone tissue engineering due to its chemical properties, similar to those of the bone mineral phase and its enhanced in vivo bioresorption. However, the biological effects of nCHA nanoparticles on cells and tissues are not sufficiently known. This study assessed the impact of exposing pre‐osteoblasts to suspensions with high doses of nCHA nanoparticles with high or low crystallinity. MC3T3‐E1 pre‐osteoblasts were cultured for 1 or 7 days in a culture medium previously exposed to CHA nanoparticles for 1 day. Control groups were produced by centrifugation for removal of bigger nCHA aggregates before exposure. Interaction of nanoparticles with the culture medium drastically changed medium composition, promoting Ca, P, and protein adsorption. Transmission Electron microscopy revealed that exposed cells were able to internalize both materials, which seemed concentrated inside endosomes. No cytotoxicity was observed for both materials, regardless of centrifugation, and the exposure did not induce alterations in the release of pro‐and anti‐inflammatory cytokines. Morphological analysis revealed strong interactions of nCHA aggregates with cell surfaces, however without marked alterations in morphological features and cytoskeleton ultrastructure. The overall in vitro biocompatibility of nCHA materials, regardless of physicochemical characteristics such as crystallinity, encourages further studies on their clinical applications.