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Decellularized human liver scaffold‐based three‐dimensional culture system facilitate hepatitis B virus infection
Author(s) -
Zhang ZhenZhen,
Xu HongMei,
Mazza Giuseppe,
Zhang MingMan,
Frenguelli Luca,
Liu QuanBo,
AlAkkad Walid,
Ren JiHua,
Zhao RuiQiu,
Ren Fang,
Chen Xin,
Huang AiLong,
Chen Juan
Publication year - 2019
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36690
Subject(s) - cccdna , decellularization , hbsag , hepatitis b virus , entecavir , cell culture , in vivo , scaffold , in vitro , virology , 3d cell culture , secretion , biology , materials science , virus , medicine , biomedical engineering , biochemistry , genetics , microbiology and biotechnology , lamivudine
Hepatitis B virus (HBV) study is hampered by lacking of idea cell model which support effective HBV infection and meanwhile recapitulate hepatocyte biology function in vivo . In this study, we developed decellularized human liver scaffolds for cell culture and further applied for HBV infection. As a result, primary human hepatocytes (PHHs) engrafted into liver scaffolds and maintained differentiation with stable albumin secretion and liver‐specific gene expression. Comparing to mono‐layer cell culture, scaffold‐based three‐dimensional (3D) culture system significantly augment HBV DNA (including cccDNA), RNA level as well as HBsAg secretion. Moreover, HepG2‐NTCP cells cultured on 3D system exhibited higher infection efficiency and longer infection period in vitro . In addition, HBV DNA level was suppressed when anti‐HBV medicine Entecavir (ETV) introduced into HepG2‐NTCP 3D system. Herein, we evaluated the potential of decellularized human liver scaffold‐based in 3D cell culture and disclosed that scaffold‐based 3D culture system can facilitate HBV infection in vitro . This 3D culture system could be further applied in HBV‐related study. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1744–1753, 2019.