Premium
Hierarchical microchannel architecture in chitosan/bioactive glass scaffolds via electrophoretic deposition positive‐replica
Author(s) -
Ghalayani Esfahani Arash,
Soleimanzade Mehdi,
Campiglio Chiara Emma,
Federici Angelica,
Altomare Lina,
Draghi Lorenza,
Boccaccini Aldo R.,
De Nardo Luigi
Publication year - 2019
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36660
Subject(s) - materials science , electrophoretic deposition , simulated body fluid , bioactive glass , chitosan , microchannel , nucleation , scaffold , biomedical engineering , composite number , nanotechnology , chemical engineering , matrix (chemical analysis) , nanocomposite , composite material , scanning electron microscope , coating , chemistry , medicine , organic chemistry , engineering
One of the main challenges in the design of scaffolds for cortical bone regeneration is mimicking the highly oriented, hierarchical structure of the native tissue in an efficient, simple, and consistent way. As a possible solution to this challenge, positive replica based on electrophoretic deposition (EPD) was here evaluated as a technique to produce organic/inorganic scaffolds with oriented micro‐porosities mimicking Haversian canals diameter and spacing. Two different sizes of 45S5 bioactive glass (BG) powders were chosen as inclusions and loaded in a chitosan matrix via EPD on micro‐patterned cathodes. Self‐standing chitosan scaffolds, with a homogeneous dispersion of BG particles and regularly‐oriented micro‐channels (ϕ = 380 ± 50 μm, inter‐channel spacing = 600 ± 40 μm), were obtained. In vitro analysis in simulated body fluid (SBF) revealed the ability to induce a deposition of a homogenous layer of hydroxyapatite (HA), with Ca/P nucleation reactions appearing kinetically favored by smaller BG particles. Cell interaction with hybrid scaffolds was evaluated in vitro with bone osteosarcoma cells (SAOS‐2). The osteoconductive potential of EPD structures was assessed by evaluating cells proliferation, viability and scaffold colonization. Results indicate that EPD is a simple yet extremely effective technique to prepare composite micro‐patterned structures and can represent a platform for the development of a new generation of bone scaffolds. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2019.