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Diverse preparation methods for small intestinal submucosa (SIS): Decellularization, components, and structure
Author(s) -
Ji Yanhui,
Zhou Jinge,
Sun Tingfang,
Tang Kai,
Xiong Zekang,
Ren Zhengwei,
Yao Sheng,
Chen Kaifang,
Yang Fan,
Zhu Fengzhao,
Guo Xiaodong
Publication year - 2019
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36582
Subject(s) - decellularization , materials science , biocompatibility , biomedical engineering , extracellular matrix , tissue engineering , biomaterial , scaffold , native tissue , nanotechnology , medicine , biology , microbiology and biotechnology , metallurgy
The native extracellular matrix (ECM) biomaterial derived from small intestinal submucosa (SIS) is widely applied in tissue engineering for tissue repair and regeneration. SIS ECM is obtained through physical and chemical methods to remove the intrinsic cells, which would otherwise cause adverse immune reactions when the SIS ECM is implanted into the host body. Several research teams have reported diverse SIS decellularization methods. However, there was no consensus on the criteria to be used for the decellularization methods for SIS and further research on the mechanism action of SIS is needed for comprehensive detection of the biological composition. In this present study, we used three reported methods to prepare SIS and compared their effects on decellularization and the remaining biological components, microstructure and cytocompatibility. SIS prepared by the three kinds of decellularization methods all achieved the recommended criteria, had good biocompatibility and retained most active components. Nevertheless, regardless of which decellularization method was used, the microstructure and bioactive components of the prepared SIS were damaged in varying degrees. We recommend that researchers need to select a decellularization method that would be appropriate to use according to their research purposes. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 689–697, 2019.