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Influence of chemically modified Luffa on the preparation of nanofiber and its biological evaluation for biomedical applications
Author(s) -
Mary Stella S.,
Vijayalakshmi U.
Publication year - 2019
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36577
Subject(s) - materials science , nanofiber , polylactic acid , fourier transform infrared spectroscopy , electrospinning , scanning electron microscope , cellulose , surface modification , composite material , polymer , chemical engineering , composite number , fiber , engineering
In the present investigation, the natural cellulose was extracted from Luffa cylindrica vegetable sponge by chemical modification. Both chemically modified and unmodified Luffa was characterized by Fourier‐transform infrared spectroscopy (FTIR), X‐ray powder diffraction (XRD), scanning electron microscopy (SEM), and energy‐dispersive X‐ray spectroscopy. The chemically modified cellulose was used for the preparation of a nanofibrous scaffold using the electrospinning method. In order to achieve the uniform and bead free fibers with desired fiber diameter the parameters such as applied voltage, tip to collector distance, solution concentration were optimized. Different ratio of hydroxyapatite (HAP): polylactic acid (PLA) such as 40:60, 50:50, 60:40, and 70:30 have been selected for the current evaluation and was compared with HAP‐treated cellulose (TC)‐PLA. With the increase in the concentration of HAP in the polymeric network, the diameter of the fiber was found to be thin with the high electric field. The functional group, phase formation and dielectric and mechanical properties of the developed nanofiber have been characterized by FTIR, XRD, mechanical property measurements, and SEM. From the results, we observed that the polymer composite developed with the ratio of 70:30 produces a bead free product with enhanced mechanical and bioactivity property by the formation of hydroxy carbonated apatite layer on the surface. All the nanofibrous scaffold fabricated with and without modification have shown good Cyto compatibility on MG‐63 Osteoblast cell lines at 48 hr. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 610–620, 2019.