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Effects of phosphatidylserine‐containing supported lipid bilayers on the polarization of macrophages
Author(s) -
Quan Hongxuan,
Kim Yongjoon,
Park Hee Chul,
Yang HyeongCheol
Publication year - 2018
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36454
Subject(s) - materials science , macrophage polarization , phosphatidylserine , biophysics , titanium , photobleaching , microbiology and biotechnology , macrophage , membrane , biochemistry , biology , phospholipid , in vitro , fluorescence , physics , quantum mechanics , metallurgy
Placement of dental implants initiates inflammatory foreign body response, in which macrophages play a central role and affect the subsequent tissue healing process such as bone formation. The purpose of this study was to fabricate phosphatidylserine (PS)‐containing supported lipid bilayers (SLBs) on a titanium surface to regulate the polarization of macrophages, a critical factor that affects following tissue healing and regeneration. The fluorescent recovery after photobleaching images showed that the percentage of PS had a critical influence on the fluidity, and 20% PS had the highest fluidity. Furthermore, more expanded and elongated cells were observed in the SLB‐coated groups. transforming growth factor‐β1 and vascular endothelial growth factor, the key cytokine markers of M2 macrophage polarization, were increased in the SLB‐coated groups, especially in the 20% PS group. Consistently, cells cultured on the SLB‐coated titanium exhibited the distribution of CD206 + , which is a M2 macrophage specific maker. The results of this study demonstrated M2 polarization of macrophages on PS‐SLB‐coated titanium discs, which suggests the application of PS‐SLB as an immune‐regulating coating material to improve tissue reactions to dental implants. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2625–2633, 2018.