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The effect of hydroxyapatite nanoparticles on adipogenic differentiation of human mesenchymal stem cells
Author(s) -
Yang Xing,
Li Yuanyuan,
Huang Qianli,
Liu Xujie,
Zhang Ranran,
Feng Qingling
Publication year - 2018
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36378
Subject(s) - adipogenesis , mesenchymal stem cell , materials science , in vitro , biocompatibility , stem cell , microbiology and biotechnology , viability assay , triglyceride , biomedical engineering , cellular differentiation , cell , nanotechnology , biology , biochemistry , gene , cholesterol , medicine , metallurgy
Due to its excellent biocompatibility, nanosized hydroxyapatite (nHA) has drawn much attention for various applications in biomedical fields. There are growing concerns about its biosecurity; however, little is known about its effects on adipogenesis. In the present study, nHA with three different sizes were synthesized, and the in vitro effects of nHA on cell proliferation and adipogenic differentiation of human mesenchymal stem cells (hMSCs) were investigated. The results clearly show that nHA does not affect the cell viability, the lipids droplets formation, triglyceride (TG) synthesis, and the expression of adipogenic marker genes/proteins of hMSCs at concentrations lower than 50 μg/mL. It is concluded that the adipogenic differentiation potential of hMSCs is not affected by nHA at noncytotoxic concentrations. These will provide a reference for the applications of nHA in biomedical fields. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1822–1831, 2018.