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Regulation of skeletal myotube formation and alignment by nanotopographically controlled cell‐secreted extracellular matrix
Author(s) -
Jiao Alex,
Moerk Charles T.,
Penland Nisa,
Perla Mikael,
Kim Jinsung,
Smith Alec S. T.,
Murry Charles E.,
Kim DeokHo
Publication year - 2018
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36351
Subject(s) - extracellular matrix , myogenesis , microbiology and biotechnology , myocyte , skeletal muscle , tissue engineering , matrix (chemical analysis) , biology , cell fusion , chemistry , cell , anatomy , biochemistry , genetics , chromatography
Skeletal muscle has a well‐organized tissue structure comprised of aligned myofibers and an encasing extracellular matrix (ECM) sheath or lamina, within which reside satellite cells. We hypothesize that the organization of skeletal muscle tissues in culture can affect both the structure of the deposited ECM and the differentiation potential of developing myotubes. Furthermore, we posit that cellular and ECM cues can be a strong determinant of myoblast fusion and morphology in 3D tissue culture environments. To test these, we utilized a thermoresponsive nanofabricated substratum to engineer anisotropic sheets of myoblasts which could then be transferred and stacked into multilayered tissues. Within such engineered tissues, we found that myoblasts rapidly sense topography and deposit structurally organized ECM proteins. Furthermore, the initial tissue structure was found to exert significant control over myoblast fusion and eventual myotube organization. These results highlight the importance of ECM structure on myoblast fusion and organization, and provide insights into substrate‐mediated control of myotube formation in the development of novel, more effective, engineered skeletal muscle tissues. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1543–1551, 2018.