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In vivo immuno‐reactivity analysis of the porous three‐dimensional chitosan/SiO 2 and chitosan/SiO 2 /hydroxyapatite hybrids
Author(s) -
Guo Mengxia,
Dong Yifan,
Xiao Jiangwei,
Gu Ruicai,
Ding Maochao,
Huang Tao,
Li Junhua,
Zhao Naru,
Liao Hua
Publication year - 2018
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36320
Subject(s) - materials science , chitosan , in vivo , biophysics , transmission electron microscopy , chemical engineering , nanotechnology , biology , microbiology and biotechnology , engineering
Inorganic/organic hybrid silica‐chitosan (CS) scaffolds have promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology. However, the precise in vivo immuno‐reactivity of silica‐CS hybrids with various compositions is still poorly defined. In this study, we fabricated the three‐dimensional (3D) interconnected porous chitosan‐silica (CS/SiO 2 ) and chitosan‐silica‐hydroxyapatite (CS/SiO 2 /HA) hybrids, through sol–gel process and 3D plotting skill, followed by the naturally or freeze drying separately. Scanning electron microscopy demonstrated the hybrids possessed the uniform geometric structure, while, transmission electron microscopy displayed nanoscale silica, or HA nanoparticles dispersed homogeneously in the CS matrix, or CS/silica hybrids. After intramuscular implantation, CS/SiO 2 and CS/SiO 2 /HA hybrids triggered a local and limited monocyte/macrophage infiltration and myofiber degeneration. Naturally dried CS/SiO 2 hybrid provoked a more severe inflammation than the freeze‐dried ones. Dendritic cells were attracted to invade into the implants embedded‐muscle, but not be activated to prime the adaptive immunity, because the absence of cytotoxic T cells and B cells in muscle received the implants. Fluorescence‐activated cell sorting (FACS) analysis indicated the implanted hybrids were incapable to initiate splenocytes activation. Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease. Thus, the present results suggest that, in vivo , 3D plotted porous CS/SiO 2 and CS/SiO 2 /HA hybrids are relatively biocompatible in vivo, which initiate a localized inflammatory procedure, instead of a systematic immune response. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1223–1235, 2018.