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An engineered tendon/ligament bioscaffold derived from decellularized and demineralized cortical bone matrix
Author(s) -
Yang JieLiang,
Yao Xuan,
Qing Quan,
Zhang Yi,
Jiang YanLin,
Ning LiangJu,
Luo JingCong,
Qin TingWu
Publication year - 2018
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36261
Subject(s) - decellularization , materials science , extracellular matrix , dbm , biomedical engineering , demineralized bone matrix , ligament , tendon , matrix (chemical analysis) , tissue engineering , anatomy , microbiology and biotechnology , medicine , biology , composite material , amplifier , optoelectronics , cmos
Demineralized bone matrix (DBM), as an extracellular matrix (ECM), has had limited use as a medical replacement although studies have reported a possibility for its use in tendon or ligament tissue engineering. To be an acid‐extracted organic matrix, DBM contains much of bone protein, with a small amount of inorganic solids and some cell debris. However, cell debris is a critical factor that triggers inflammatory reaction in clinical reconstructions using ECM. In this study, we used a protocol incorporating the use of detergent with nuclease treatment to prepare decellularized DBM (DCDBM). DNA quantification analysis and histological observation confirmed that cells were completely removed from DBM. The inherent ultrastructure of DBM was well preserved after decellularization as observed through scanning electron microscopy. Additionally, calcium and phosphorus were absent and the specific functional groups of collagen remained after decellularization. Moreover, 79.71% of the tensile strength of DBM was retained and the viscoelastic properties were similar to the ligament. Furthermore, DCDBM promoted the adhesion and proliferation of NIH‐3T3 fibroblasts in vitro and triggered less inflammation response at 12 weeks subcutaneous implantation in a rat model. These results demonstrate that the DCDBM has the potential to be used for tendon and ligament replacement. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 468–478, 2018.