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Direct surface modification of metallic biomaterials via tyrosine oxidation aiming to accelerate the re‐endothelialization of vascular stents
Author(s) -
Kakinoki Sachiro,
Takasaki Kensuke,
Mahara Atsushi,
Ehashi Tomo,
Hirano Yoshiaki,
Yamaoka Tetsuji
Publication year - 2018
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36258
Subject(s) - restenosis , surface modification , materials science , bare metal , adhesion , biomaterial , bare metal stent , biomedical engineering , cell adhesion , endothelial progenitor cell , stent , endothelial stem cell , chemistry , nanotechnology , biochemistry , in vitro , drug eluting stent , medicine , composite material
Rapid in‐situ re‐endothelialization of coronary stents is one of the most effective approaches to inhibit late thrombosis and restenosis. Strut surfaces allowing excellent adhesion and migration of endothelial cells and endothelial progenitor cells may accelerate in‐situ re‐endothelialization. Here, a well‐known endothelial cell adhesive peptide, Arg‐Glu‐Asp‐Val (REDV), was directly immobilized onto metallic surfaces by means of single‐step tyrosine oxidation with copper chloride (II) and hydrogen peroxide, which we recently reported as a new biomaterial modification technique. REDV immobilization on a 316L stainless steel plate improved endothelial cell adhesion and effectively suppressed platelet adhesion in vitro . In addition, a Co‐Cr stent immobilized with Ac‐Tyr‐Gly‐Gly‐Gly‐Arg‐Glu‐Asp‐Val (Y‐REDV) was implanted into a rabbit abdominal aorta. On 7 days postimplantation, 80% of the strut surface of the Y‐REDV‐immobilized stent was covered by a thin neointimal layer and was similar in appearance to native endothelium. Restenosis and late thrombosis were not observed in the Y‐REDV‐immobilized stent for 42 days. These findings suggest that direct immobilization of Y‐REDV peptide onto metallic biomaterials by tyrosine oxidation is effective for promoting in‐situ re‐endothelialization in vascular stents. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 491–499, 2018.