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Crosslinked basement membrane‐based coatings enhance glucose sensor function and continuous glucose monitoring in vivo
Author(s) -
Klueh Ulrike,
Ludzinska Izabela,
Czajkowski Caroline,
Qiao Yi,
Kreutzer Donald L.
Publication year - 2018
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36206
Subject(s) - materials science , in vivo , continuous glucose monitoring , coating , glutaraldehyde , biomedical engineering , biocompatible material , membrane , in vitro , nanotechnology , diabetes mellitus , chromatography , medicine , biochemistry , chemistry , type 1 diabetes , biology , microbiology and biotechnology , endocrinology
Overcoming sensor‐induced tissue reactions is an essential element of achieving successful continuous glucose monitoring (CGM) in the management of diabetes, particularly when used in closed loop technology. Recently, we demonstrated that basement membrane (BM)‐based glucose sensor coatings significantly reduced tissue reactions at sites of device implantation. However, the biocompatible BM‐based biohydrogel sensor coating rapidly degraded over a less than a 3‐week period, which effectively eliminated the protective sensor coating. In an effort to increase the stability and effectiveness of the BM coating, we evaluated the impact of crosslinking BM utilizing glutaraldehyde as a crosslinking agent, designated as X‐Cultrex. Sensor performance (nonrecalibrated) was evaluated for the impact of these X‐Cultrex coatings in vitro and in vivo . Sensor performance was assessed over a 28‐day time period in a murine CGM model and expressed as mean absolute relative difference (MARD) values. Tissue reactivity of Cultrex‐coated, X‐Cultrex‐coated, and uncoated glucose sensors was evaluated over a 28‐day time period in vivo using standard histological techniques. These studies demonstrated that X‐Cultrex‐based sensor coatings had no effect on glucose sensor function in vitro. In vivo , glucose sensor performance was significantly enhanced following X‐Cultrex coating throughout the 28‐day study. Histological evaluations of X‐Cultrex‐treated sensors demonstrated significantly less tissue reactivity when compared to uncoated sensors. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 7–16, 2018.