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Response of preosteoblasts to titanium with periodic micro/nanometer scale grooves produced by femtosecond laser irradiation
Author(s) -
Chen Peng,
Miyake Masayoshi,
Tsukamoto Masahiro,
Tsutsumi Yusuke,
Hanawa Takao
Publication year - 2017
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36202
Subject(s) - materials science , femtosecond , osseointegration , nanometre , laser , irradiation , biomaterial , titanium , nanotechnology , adhesion , nanoscopic scale , biomedical engineering , optics , composite material , implant , medicine , physics , surgery , nuclear physics , metallurgy
To investigate the cellular response to designed topography in vitro , we studied the adhesion, proliferation, osteogenic differentiation, and calcification of mouse preosteoblasts (MC3T3‐E1) cultured on titanium (Ti) surfaces with periodic micrometer scale grooves containing nanometer scale ripples in the vertical direction fabricated by single‐shot, femtosecond laser irradiation (fsTi). The surface composition and chemical state of fsTi were almost the same as those of mirror‐polished Ti without femtosecond laser irradiation (mTi). Cells cultured on fsTi were highly aligned, whereas the cell proliferation rate on fsTi was less than that on mTi. Higher gene expressions of Spp1 and Bglap1 were detected in cells cultured on fsTi than those on mTi, indicating that the periodic micro/nanometer scale grooves topography promoted osteogenic differentiation and calcification. This initial activation of osteoinduction on fsTi generated calcified deposits that were thicker and larger than those on mTi and hence, osteoconductivity was promoted on fsTi. Our findings indicate that femtosecond laser irradiation is a technique with potential for controlling biomaterial–cell interfaces and, in particular, the promotion of osseointegration of Ti. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3456–3464, 2017.