In vitro  basic fibroblast growth factor (bFGF) delivery using an antithrombogenic 2‐methacryloyloxyethyl phosphorylcholine (MPC) polymer coated with a micropatterned diamond‐like carbon (DLC) film | Zendy

Bito Kenta | Zendy; Hasebe Terumitsu | Zendy; Maegawa Shunto | Zendy; Maeda Tomoki | Zendy; Matsumoto Tomohiro | Zendy; Suzuki Tetsuya | Zendy; Hotta Atsushi | Zendy

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In vitro basic fibroblast growth factor (bFGF) delivery using an antithrombogenic 2‐methacryloyloxyethyl phosphorylcholine (MPC) polymer coated with a micropatterned diamond‐like carbon (DLC) film

Author(s) -

Bito Kenta,

Hasebe Terumitsu,

Maegawa Shunto,

Maeda Tomoki,

Matsumoto Tomohiro,

Suzuki Tetsuya,

Hotta Atsushi

Publication year - 2017

Publication title -

journal of biomedical materials research part a

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.849

H-Index - 150

eISSN - 1552-4965

pISSN - 1549-3296

DOI - 10.1002/jbm.a.36201

Subject(s) - materials science , basic fibroblast growth factor , polymer , phosphorylcholine , diamond like carbon , drug delivery , biomedical engineering , nanotechnology , composite material , growth factor , thin film , chemistry , biochemistry , medicine , receptor

In this study, a newly designed drug‐release platform composed of an antithrombogenic 2‐methacryloyloxyethyl phosphorylcholine (MPC) polymer was introduced, which was impregnated with basic fibroblast growth factor (bFGF) (bFGF/MPC polymer) to enhance the endothelial cell activation. The platform was also coated with an ultrathin micropatterned diamond‐like carbon (DLC) film (DLC/bFGF/MPC polymer) to precisely control the drug release rate and the cell compatibility. The resulting DLC/bFGF/MPC polymer could effectively prolong the bFGF release rate by depositing the micropatterned DLC. The number of adherent platelets on the DLC/bFGF/MPC polymer was significantly lower (about 1/14) than that on a currently used stent made of stainless steel (SUS316L), indicating the enhanced antithrombogenicity in the DLC/bFGF/MPC polymer. The proliferation of endothelial cells on the DLC/bFGF/MPC polymer and the DLC/MPC polymer (without bFGF) were also examined. It was found that the optical density of HUVEC on the DLC/bFGF/MPC polymer determined by WST‐8 assay was higher by 25%than that on the DLC/MPC polymer (without bFGF) measured after 72 h of incubation. Our results suggest that the released bFGF that contributes to the expression of other growth factors results in the early proliferation of the HUVEC on the DLC/bFGF/MPC polymer. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3384–3391, 2017.

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