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Osteogenesis for postoperative temporal bone defects using human ear adipose‐derived stromal cells and tissue engineering: An animal model study
Author(s) -
Kim Yeon Ju,
Park Seung Gu,
Shin Beomyong,
Kim Jangho,
Kim Seung Won,
Choo OakSung,
Yin Xiang Yun,
Min Byoung Hyun,
Choung YunHoon
Publication year - 2017
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36194
Subject(s) - mastoidectomy , polycaprolactone , materials science , biomedical engineering , temporal bone , stromal cell , middle ear , adipose tissue , otitis , bulla (seal) , anatomy , medicine , surgery , pathology , cholesteatoma , lung , composite material , endocrinology , polymer
Mastoidectomy, the removal of infected mastoid bones, is a common surgical procedure for the treatment of chronic otitis media. Persistent and recurrent otorrhea and accumulation of keratin debris following open cavity mastoidectomy are still bothersome issues for both patients and otologists. In this study, we used human ear adipose‐derived stromal cells (hEASCs) in combination with polycaprolactone (PCL) scaffolds and osteogenic differentiation medium (ODM) to regenerate temporal bone defects. The hEASCs showed stem cell phenotypes, and these characteristics were maintained up to passage 5. Mastoid bulla and cranial bone defects were induced in Sprague‐Dawley rats using AgNO 3 and burr hole drilling, respectively, and the rats were then divided into five groups: (1) control, (2) hEASCs, (3) hEASCs + ODM, (4) hEASCs + PCL scaffolds, and (5) hEASCs + PCL scaffolds + ODM. Osteogenesis was evaluated by micro‐computed tomography and histology. Compared with the control group, the groups transplanted with hEASCs and PCL scaffolds had significantly higher bone formation along the periphery of the mastoid bulla area. Moreover, ODM synergistically enhanced bone formation in mastoid bulla defects. Our results suggest that combining hEASCs with PCL scaffolds represents a promising method for anatomical and functional reconstruction of postoperative temporal bone defects following mastoidectomy. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3493–3501, 2017.