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Quercetin and alendronate multi‐functionalized materials as tools to hinder oxidative stress damage
Author(s) -
Forte Lucia,
Torricelli Paola,
Boanini Elisa,
Rubini Katia,
Fini Milena,
Bigi Adriana
Publication year - 2017
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36192
Subject(s) - quercetin , materials science , osteoclast , oxidative stress , osteoblast , bioavailability , biophysics , nuclear chemistry , pharmacology , in vitro , biochemistry , chemistry , medicine , antioxidant , biology
Abstract In spite of its remarkable anti‐oxidant, anti‐inflammatory, anti‐cancer properties and its possible inhibition activity towards bone resorption, quercetin therapeutic use is limited by its poor bioavailability. Herein we developed a new multifunctionalized system for the local administration of quercetin and alendronate, one of the most potent anti‐osteoporotic drugs, with the aim to get a material with enhanced properties. To this purpose we loaded quercetin on hydroxyapatite functionalized with alendronate, as well as on hydroxyapatite. Characterization was performed by means of X‐ray diffraction, FT‐IR and Raman spectroscopies, thermogravimetric and spectrophotometric analyses. Loading of quercetin from hydro‐alcoholic solution increased with time and reached a constant value of about 5 weight% on both substrates, without causing significant structural and morphological modifications. Quercetin functionalized materials exhibit relevant anti‐oxidant properties, in agreement with their high radical scavenging activity, and a quercetin sustained release in phosphate buffer. In vitro osteoblast and osteoclast co‐culture in a microenvironment altered by oxidative stress shows that both alendronate and quercetin significantly reduce osteoclast viability, whereas they are able to counteract the negative effect of oxidative stress on osteoblast viability and differentiation, suggesting that their relative amount in the functionalized materials can be utilized to tailor bone cells response. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3293–3303, 2017.