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The extracts of bredigite bioceramics enhanced the pluripotency of human dental pulp cells
Author(s) -
Chen Lihong,
Liu Lu,
Wu Chengtie,
Yang Ruiqi,
Chang Jiang,
Wei Xi
Publication year - 2017
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36191
Subject(s) - sox2 , materials science , regenerative medicine , microbiology and biotechnology , dental pulp stem cells , basic fibroblast growth factor , stem cell , tissue engineering , biomedical engineering , cell growth , biology , growth factor , embryonic stem cell , biochemistry , medicine , receptor , gene
Biomaterials have a profound effect on tissue engineering and regenerative medicine, but few studies have reported the role of extracts from bioceramics in the regulation of stem cell pluripotency. The present study investigated the effects of bioceramics extracts, including silicate bredigite (Ca 7 MgSi 4 O 16 ) and conventional β‐tricalcium phosphate (β‐TCP), on the pluripotency and the multilineage differentiation potential of human dental pulp cells (hDPCs). Basic fibroblast growth factor (bFGF), which is a known regulator of hDPCs pluripotency, was used as a reference. Bredigite extracts significantly promoted cell growth, proliferation, TERT expression and maintained hDPCs in a presenescent state. The extracts of bredigite significantly up‐regulated the expression of pluripotency‐related genes such as Stro1, Oct4 and Sox2, and further promoted the multilineage differentiation of hDPCs after odontogenic/adipogenic induction. The stimulation of bredigite extracts on hDPCs pluripotency was comparable to that of bFGF, whereas β‐TCP extracts lacked these properties. Our results suggested for the first time that bredigite extracts enhance the pluripotency of dental‐derived stem cells, paving the way for extended applications in regenerative medicine. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3465–3474, 2017.