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Control of cell growth on 3D‐printed cell culture platforms for tissue engineering
Author(s) -
Tan Zhikai,
Liu Tong,
Zhong Juchang,
Yang Yikun,
Tan Weihong
Publication year - 2017
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36188
Subject(s) - materials science , tissue engineering , biomedical engineering , plga , 3d printed , biocompatible material , connective tissue , cell growth , cell adhesion , 3d printing , 3d cell culture , cell culture , nanotechnology , adhesion , composite material , chemistry , engineering , biology , biochemistry , nanoparticle , genetics
Biocompatible tissue growth has excellent prospects for tissue engineering. These tissues are built over scaffolds, which can influence aspects such as cell adhesion, proliferation rate, morphology, and differentiation. However, the ideal 3D biological structure has not been developed yet. Here, we applied the electro‐hydrodynamic jet (E‐jet) 3D printing technology using poly‐(lactic‐co‐glycolic acid, PLGA) solution to print varied culture platforms for engineered tissue structures. The effects of different parameters (electrical voltage, plotting speed, and needle sizes) on the outcome were investigated. We compared the biological compatibility of the 3D printed culture platforms with that of random fibers. Finally, we used the 3D‐printed PLGA platforms to culture fibroblasts, the main cellular components of loose connective tissue. The results show that the E‐jet printed platforms could guide and improve cell growth. These highly aligned fibers were able to support cellular alignment and proliferation. Cell angle was consistent with the direction of the fibers, and cells cultured on these fibers showed a much faster migration, potentially enhancing wound healing performance. Thus, the potential of this technology for 3D biological printing is large. This process can be used to grow biological scaffolds for the engineering of tissues. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3281–3292, 2017.