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Low‐thrombogenic fibrin‐heparin coating promotes in vitro endothelialization
Author(s) -
Kaplan Ondřej,
Hierlemann Teresa,
Krajewski Stefanie,
Kurz Julia,
Nevoralová Martina,
Houska Milan,
Riedel Tomáš,
Riedelová Zuzana,
Zárubová Jana,
Wendel Hans P.,
Brynda Eduard
Publication year - 2017
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36152
Subject(s) - fibrin , materials science , umbilical vein , thrombin , fibrinogen , biomedical engineering , coating , heparin , platelet , endothelium , in vitro , substrate (aquarium) , thrombus , biophysics , nanotechnology , immunology , biochemistry , surgery , chemistry , medicine , biology , ecology
Long‐term performance of implanted cardiovascular grafts can be ensured if living endothelium overgrows their surface. Surface modifications to implants are therefore being sought that can encourage endothelialization while preventing thrombus formation until the natural endothelium is formed. In the present study, heparin was covalently attached to a fibrin mesh grown from a polyvinyl chloride (PVC) substrate surface by the catalytic action of surface immobilized thrombin on a fibrinogen solution. The coating prevented platelet activation, thrombin generation and clot formation, and reduced inflammatory reactions when exposed to fresh human whole blood circulating in a Chandler loop model. In addition, in vitro seeded human umbilical vein and human saphenous vein endothelial cells showed considerably enhanced attachment and proliferation on the coating. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2995–3005, 2017.