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Use of lecithin to control fiber morphology in electrospun poly (ɛ‐caprolactone) scaffolds for improved tissue engineering applications
Author(s) -
Coverdale Benjamin D.M.,
Gough Julie E.,
Sampson William W.,
Hoyland Judith A.
Publication year - 2017
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36139
Subject(s) - materials science , scaffold , tissue engineering , biocompatibility , biomedical engineering , caprolactone , electrospinning , porosity , mesenchymal stem cell , lecithin , chemical engineering , composite material , polymer , chromatography , chemistry , microbiology and biotechnology , medicine , engineering , copolymer , metallurgy , biology
We elucidate the effects of incorporating surfactants into electrospun poly (ɛ‐caprolactone) (PCL) scaffolds on network homogeneity, cellular adherence and osteogenic differentiation. Lecithin was added with a range of concentrations to PCL solutions, which were electrospun to yield functionalized scaffolds. Addition of lecithin yielded a dose‐dependent reduction in scaffold hydrophobicity, whilst reducing fiber width and hence increasing specific surface area. These changes in scaffold morphology were associated with increased cellular attachment of Saos‐2 osteoblasts 3‐h postseeding. Furthermore, cells on scaffolds showed comparable proliferation over 14 days of incubation to TCP controls. Through model‐based interpretation of image analysis combined with gravimetric estimates of porosity, lecithin is shown to reduce scaffold porosity and mean pore size. Additionally, lecithin incorporation is found to reduce fiber curvature, resulting in increased scaffold specific elastic modulus. Low concentrations of lecithin were found to induce upregulation of several genes associated with osteogenesis in primary mesenchymal stem cells. The results demonstrate that functionalization of electrospun PCL scaffolds with lecithin can increase the biocompatibility and regenerative potential of these networks for bone tissue engineering applications. © 2017 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2865–2874, 2017.