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The effect of riboflavin/UVA cross‐linking on anti‐degeneration and promoting angiogenic capability of decellularized liver matrix
Author(s) -
Xiang Junxi,
Liu Peng,
Zheng Xinglong,
Dong Dinghui,
Fan Shujuan,
Dong Jian,
Zhang Xufeng,
Liu Xuemin,
Wang Bo,
Lv Yi
Publication year - 2017
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36126
Subject(s) - decellularization , materials science , biomedical engineering , matrix (chemical analysis) , angiogenesis , in vivo , tissue engineering , composite material , medicine , biology , cancer research , microbiology and biotechnology
Weak mechanical property and unstable degradation rate limited the application of decellularized liver matrix in tissue engineering. The aim of this study was to explore a new method for improving the mechanical properties, anti‐degeneration and angiogenic capability of decellularized liver matrix. This was achieved by a novel approach using riboflavin/ultraviolet A treatment to induce collagen cross‐linking of decellularized matrix. Histological staining and scanning electron microscope showed that the diameter of cross‐linked fibers significantly increased compared with the control group. The average peak load and Young's modulus of decellularized matrix were obviously improved after cross‐linking. Then we implanted the modified matrix into the rat hepatic injury model to test the anti‐degeneration and angiogenic capability of riboflavin/UVA cross‐linked decellularized liver scaffolds in vivo . The results indicated that cross‐linked scaffolds degrade more slowly than those in the control group. In the experiment group, average microvessel density in the implanted matrix was higher than that in the control group since the first week after implantation. In conclusion, we initiated the method to improve the biomechanical properties of decellularized liver scaffolds by riboflavin/UVA cross‐linking, and more importantly, its improvement on anti‐degeneration and angiogenesis was identified. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2662–2669, 2017.