Premium
Cationic osteogenic peptide P15‐CSP coatings promote 3‐D osteogenesis in poly(epsilon‐caprolactone) scaffolds of distinct pore size
Author(s) -
Li Xian,
Ghavidel Mehr Nima,
GuzmánMorales Jessica,
Favis Basil D.,
De Crescenzo Gregory,
Yakandawala Nandadeva,
Hoemann Caroline D.
Publication year - 2017
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36082
Subject(s) - biomineralization , materials science , scaffold , mineralization (soil science) , mesenchymal stem cell , sponge , biomedical engineering , biophysics , chemical engineering , chemistry , organic chemistry , pathology , medicine , botany , nitrogen , engineering , biology
P15‐CSP is a biomimetic cationic fusion peptide that stimulates osteogenesis and inhibits bacterial biofilm formation when coated on 2‐D surfaces. This study tested the hypothesis that P15‐CSP coatings enhance 3‐D osteogenesis in a porous but otherwise hydrophobic poly‐(ɛ‐caprolactone) (PCL) scaffold. Scaffolds of 84 µm and 141 µm average pore size were coated or not with Layer‐by‐Layer polyelectrolytes followed by P15‐CSP, seeded with adult primary human mesenchymal stem cells (MSCs), and cultured 10 days in proliferation medium, then 21 days in osteogenic medium. Atomic analyses showed that P15‐CSP was successfully captured by LbL. After 2 days of culture, MSCs adhered and spread more on P15‐CSP coated pores than PCL‐only. At day 10, all constructs contained nonmineralized tissue. At day 31, all constructs became enveloped in a “skin” of tissue that, like 2‐D cultures, underwent sporadic mineralization in areas of high cell density that extended into some 141 µm edge pores. By quantitative histomorphometry, 2.5‐fold more tissue and biomineral accumulated in edge pores versus inner pores. P15‐CSP specifically promoted tissue‐scaffold integration, fourfold higher overall biomineralization, and more mineral deposits in the outer 84 µm and inner 141 µm pores than PCL‐only ( p < 0.05). 3‐D Micro‐CT revealed asymmetric mineral deposition consistent with histological calcium staining. This study provides proof‐of‐concept that P15‐CSP coatings are osteoconductive in PCL pore surfaces with 3‐D topography. Biomineralization deeper than 150 µm from the scaffold edge was optimally attained with the larger 141 µm peptide‐coated pores. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2171–2181, 2017.