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Potentiating bioactivity of BMP ‐2 by polyelectrolyte complexation with sulfonated polyrotaxanes to induce rapid bone regeneration in a mouse calvarial defect
Author(s) -
Terauchi Masahiko,
Inada Takasuke,
Kanemaru Tomoki,
Ikeda Go,
Tonegawa Asato,
Nishida Kei,
Arisaka Yoshinori,
Tamura Atsushi,
Yamaguchi Satoshi,
Yui Nobuhiko
Publication year - 2017
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36016
Subject(s) - bone morphogenetic protein 2 , materials science , ethylene glycol , regeneration (biology) , in vivo , biomedical engineering , biophysics , bone morphogenetic protein , bone tissue , in vitro , microbiology and biotechnology , chemistry , biochemistry , organic chemistry , biology , medicine , gene
Bone reconstruction is a challenging issue in the regeneration of surgically removed bone and disease‐related bone defects. Although bone morphogenetic protein‐2 (BMP‐2) has received considerable attention as a bone regeneration inducer, a high dose of BMP‐2 is typically required due to its short life‐time under in vivo conditions. We have proposed a method to enhance the osteogenetic differentiation ability of BMP‐2 in vitro that is based on supramolecular polyelectrolyte complexation with sulfonated polyrotaxanes (PRXs) consisting of sulfopropyl ether (SPE)‐modified α‐cyclodextrins threaded along a poly(ethylene glycol) chain capped with terminal bulky stopper molecules. In this study, we evaluated the in vivo bone regeneration ability of the SPE‐PRX/BMP‐2 complexes in a mouse calvarial defect model in comparison to free BMP‐2 and heparin/BMP‐2 complexes. The regenerated bone area was determined by X‐ray computed microtomography, and the mice implanted with sulfonated PRX/BMP‐2 complexes exhibited rapid and significant bone regeneration compared to those implanted with free BMP‐2 and heparin/BMP‐2 complexes. We concluded that the sulfonated PRX/BMP‐2 complexes are a promising candidate for clinical bone regeneration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1355–1363, 2017.