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Interactions of human embryonic stem cell‐derived cardiovascular progenitor cells with immobilized extracellular matrix proteins
Author(s) -
Lu Jizhen,
Kaestle Katrin,
Huang Jijun,
Liu Qiao,
Zhang Peng,
Gao Ling,
Gardiner James,
Thissen Helmut,
Yang HuangTian
Publication year - 2017
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.36005
Subject(s) - fibronectin , extracellular matrix , laminin , microbiology and biotechnology , materials science , embryonic stem cell , progenitor cell , decellularization , tissue engineering , regeneration (biology) , stem cell , biomedical engineering , biochemistry , biology , medicine , gene
Human embryonic stem cell‐derived cardiovascular progenitor cells (hESC‐CVPCs) hold great promise for cell‐based therapies of heart diseases. However, little is known about their niche microenvironment and in particular the required extracellular matrix (ECM) components. Here we screened combinations of surface‐immobilized ECM proteins to identify substrates that support the attachment and survival of hESC‐CVPCs. Covalent immobilization of ECM proteins laminin (Lm), fibronectin (Fn), collagen I (CI), collagen III (CIII), and collagen IV (CIV) in multiple combinations and concentrations was achieved by reductive amination on transparent acetaldehyde plasma polymer (AAPP) interlayer coatings. We identified that CI, CIII, CIV, and Fn and their combinations were important for hESC‐CVPC attachment and survival, while Lm was dispensable. Moreover, for coatings displaying single ECM proteins, CI and CIII performed better than CIV and Fn, while coatings displaying the combined ECM proteins CIII + CIV and Fn + CIII + CIV at 100 µg/mL were comparable to Matrigel in regard to supporting hESC‐CVPC attachment and viability. Our results identify ECM proteins required for hESC‐CVPCs and demonstrate that coatings displaying multiple immobilized ECM proteins offer a suitable microenvironment for the attachment and survival of hESC‐CVPCs. This knowledge contributes to the development of approaches for maintaining hESC‐CVPCs and therefore to advances in cardiovascular regeneration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1094–1104, 2017.