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Polyurethane conjugating TGF‐β on surface impacts local inflammation and endoplasmic reticulum stress in skeletal muscle
Author(s) -
Shi Dandan,
Xiao Jiangwei,
Gu Ruicai,
Wu Gang,
Liao Hua
Publication year - 2017
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35999
Subject(s) - materials science , in vivo , biocompatibility , endoplasmic reticulum , biophysics , transforming growth factor , quartz crystal microbalance , polymer , in vitro , regeneration (biology) , biomedical engineering , inflammation , microbiology and biotechnology , biochemistry , chemistry , immunology , biology , medicine , organic chemistry , composite material , adsorption , metallurgy
The synthesized short peptide‐polymers would provide key functions for tissue regeneration and repair, through enriching bioactive molecules on polymers or releasing these molecules pre‐conjugated on the materials. We have developed a degradable polyurethane (PU) bearing HSNGLPL peptide, which has affinity binding ability to transforming growth factor‐betas (TGF‐β). For deeply understanding spatial release of TGF‐β from the PU polymers and its localized bioactivity, quartz crystal microbalance (QCM) and Elisa test were used to verify TGF‐β binding capacities in vitro and in vivo . The PU polymers, with or without pre‐conjugating of TGF‐β, were implanted into gastronomies muscle (GN) of C57BL/6 mice, for addressing TGF‐β release from the polymers and its bio‐regulating function in vivo . QCM result shows that PU bearing HSNGLPL peptide has affinity binding ability to TGF‐β in vitro . Intramuscular implanting experiment further supports the enrichment efficiency of TGF‐β on PU polymers in vivo . The detecting data involving intramuscular inflammatory infiltration triggered by the implants, myofiber regeneration, muscular fibrosis degree, and activation of endoplasmic reticulum stress (ER stress), evidence TGF‐β can be released from PU polymers, and exerts regulating effects on the material‐induced inflammation. Thus, our present results suggest it is feasible to improve biocompatibility of PU polymers in vivo , by pre‐bearing bioactive molecules on materials before the implanting. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1156–1165, 2017.