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Cell adhesive peptides functionalized on CoCr alloy stimulate endothelialization and prevent thrombogenesis and restenosis
Author(s) -
Castellanos Maria Isabel,
GuillemMarti Jordi,
MasMoruno Carlos,
DíazRicart Maribel,
Escolar Ginés,
Ginebra Maria Pau,
Gil Francisco Javier,
Pegueroles Marta,
Manero Jose María
Publication year - 2017
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35988
Subject(s) - thrombogenicity , surface modification , adhesion , materials science , cell adhesion , covalent bond , platelet , peptide , biophysics , biomedical engineering , biochemistry , chemistry , medicine , biology , immunology , organic chemistry , composite material
Immobilization of bioactive peptide sequences on CoCr surfaces is an effective route to improve endothelialization, which is of great interest for cardiovascular stents. In this work, we explored the effect of physical and covalent immoblization of RGDS, YIGSR and their equimolar combination peptides on endothelial cells (EC) and smooth muscle cell (SMC) adhesion and on thrombogenicity. We extensively investigated using RT‐qPCR, the expression by ECs cultured on functionalised CoCr surfaces of different genes. Genes relevant for adhesion (ICAM‐1 and VCAM‐1), vascularization (VEGFA, VEGFR‐1 and VEGFR‐2) and anti‐thrombogenicity (tPA and eNOS) were over‐expressed in the ECs grown to covalently functionalized CoCr surfaces compared to physisorbed and control surfaces. Pro‐thrombogenic genes expression (PAI‐1 and vWF) decreased over time. Cell co‐cultures of ECs/SMCs found that functionalization increased the amount of adhered ECs onto modified surfaces compared to plain CoCr, independently of the used peptide and the strategy of immobilization. SMCs adhered less compared to ECs in all surfaces. All studied peptides showed a lower platelet cell adhesion compared to TCPS. Covalent functionalization of CoCr surfaces with an equimolar combination of RGDS and YIGSR represented prevailing strategy to enhance the early stages of ECs adhesion and proliferation, while preventing SMCs and platelet adhesion. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 973–983, 2017.