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Gelatin/chondroitin sulfate nanofibrous scaffolds for stimulation of wound healing: In‐vitro and in‐vivo study
Author(s) -
PezeshkiModaress Mohamad,
Mirzadeh Hamid,
Zandi Mojgan,
RajabiZeleti Sareh,
Sodeifi Niloofar,
Aghdami Nasser,
Mofrad Mohammad R. K.
Publication year - 2017
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35890
Subject(s) - gelatin , materials science , electrospinning , chondroitin sulfate , tissue engineering , biomedical engineering , wound healing , in vivo , glycosaminoglycan , fibroblast , hyaluronic acid , nanofiber , in vitro , nanotechnology , chemistry , composite material , polymer , anatomy , biochemistry , medicine , surgery , biology , microbiology and biotechnology
In this research, fabrication of gelatin/chondroitin sulfate (GAG) nanofibrous scaffolds using electrospinning technique for skin tissue engineering was studied. The influence of GAG content on chemical, physical, mechanical and biological properties of the scaffolds were investigated. Human dermal fibroblast (HDF) cells were cultured and bioactivity of electrospun gelatin/GAG scaffolds for skin tissue engineering was assayed. Biological results illustrated that HDF cells attached and spread well on gelatin/GAG nanofibrous scaffolds displaying spindle‐like shapes and stretching. MTS assay was performed to evaluate the cell proliferation on electrospun gelatin/GAG scaffolds. The results confirmed the influence of GAG content as well as the nanofibrous structure on cell proliferation and attachment of substrates. The gelatin/GAG nanofibrous scaffolds with the desired thickness for in‐vivo evaluations were used on the full‐thickness wounds. Pathobiological results showed that cell loaded gelatin/GAG scaffolds significantly accelerated wounds healing. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2020–2034, 2017.