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Competitive colonization of prosthetic surfaces by staphylococcus aureus and human cells
Author(s) -
PérezTanoira Ramón,
Han Xia,
Soininen Antti,
Aarnisalo Antti A.,
Tiainen VeliMatti,
Eklund Kari K.,
Esteban Jaime,
Kinnari Teemu J.
Publication year - 2017
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35863
Subject(s) - staphylococcus aureus , biomaterial , adhesion , materials science , microbiology and biotechnology , flow cytometry , in vitro , cell adhesion , colonization , cell , biofilm , bacteria , nanotechnology , biology , immunology , biochemistry , genetics , composite material
Implantation of a biomaterial provides an adhesion substratum both to host cell integration and to contaminating bacteria. We studied simultaneous competitive adhesion of Staphylococcus aureus in serial 1:10 dilutions of 10 8 colony forming units (CFU)/mL and human osteogenic sarcoma (SaOS‐2) or primary osteoblast (hOB) cells, both 1x10 5 cells/mL, to the surfaces of titanium, polydimethylsiloxane and polystyrene. The bacterial adherence and human cell proliferation, cytotoxicity and production of reactive oxygen species (ROS) were studied using fluorometric (fluorescent microscopy and flow cytometry) and colorimetric methods (MTT, LDH and crystal violet). The bacterial cell viability was also evaluated using the drop plate method. The presence of bacteria resulted in reduced adherence of human cells to the surface of the biomaterials, increased production of ROS, and into increased apoptosis. On the other hand, the presence of either type of human cells was associated with a reduction of bacterial colonization of the biomaterial with Staphylococcus aureus . These results suggest that increasing colonization of the biomaterial surface in vitro by one negatively affects colonization by the other. Host cell integration to an implant surface reduces bacterial contamination, which opens novel opportunities for the design of infection‐resistant biomaterials in current implantology and future regenerative medicine. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 62–72, 2017.