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Surface chemistry regulates the sensitivity and tolerability of osteoblasts to various magnitudes of fluid shear stress
Author(s) -
Li Yan,
Wang Jinfeng,
Xing Juan,
Wang Yuanliang,
Luo Yanfeng
Publication year - 2016
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35848
Subject(s) - tolerability , biophysics , focal adhesion , materials science , mechanotransduction , cell adhesion , cell growth , actin , cell , chemistry , microbiology and biotechnology , biochemistry , biology , pharmacology , adverse effect
Abstract Scaffolds provide a physical support for osteoblasts and act as the medium to transfer mechanical stimuli to cells. To verify our hypothesis that the surface chemistry of scaffolds regulates the perception of cells to mechanical stimuli, the sensitivity and tolerability of osteoblasts to fluid shear stress (FSS) of various magnitudes (5, 12, 20 dynes/cm 2 ) were investigated on various surface chemistries (–OH, –CH 3 , –NH 2 ), and their follow‐up effects on cell proliferation and differentiation were examined as well. The sensitivity was characterized by the release of adenosine triphosphate (ATP), nitric oxide (NO) and prostaglandin E 2 (PGE 2 ) while the tolerability was by cellular membrane integrity. The cell proliferation was characterized by S‐phase cell fraction and the differentiation by ALP activity and ECM expression (fibronectin and type I collagen). As revealed, osteoblasts demonstrated higher sensitivity and lower tolerability on OH and CH 3 surfaces, yet lower sensitivity and higher tolerability on NH 2 surfaces. Observations on the focal adhesion formation, F‐actin organization and cellular orientation before and after FSS exposure suggest that the potential mechanism lies in the differential control of F‐actin organization and focal adhesion formation by surface chemistry, which further divergently mediates the sensitivity and tolerability of ROBs to FSS and the follow‐up cell proliferation and differentiation. These findings are essentially valuable for design/selection of desirable surface chemistry to orchestrate with FSS stimuli, inducing appropriate cell responses and promoting bone formation. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2978–2991, 2016.