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Human mesenchymal stromal cells response to biomimetic octacalcium phosphate containing strontium
Author(s) -
Birgani Zeinab Tahmasebi,
Malhotra Angad,
van Blitterswijk Clemens A.,
Habibovic Pamela
Publication year - 2016
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35725
Subject(s) - octacalcium phosphate , bone sialoprotein , mesenchymal stem cell , osteopontin , materials science , alkaline phosphatase , strontium , bone morphogenetic protein 2 , stromal cell , phosphate , calcium , human bone , bone formation , biomedical engineering , microbiology and biotechnology , osteocalcin , chemistry , biochemistry , biology , in vitro , endocrinology , cancer research , medicine , metallurgy , enzyme , organic chemistry
The incorporation of bioinorganics into synthetic biomaterials is a promising approach to improve the biological performance of bone graft substitutes, while still retaining their synthetic nature. Among these bioinorganics, strontium ions (Sr 2+ ) have reported enhanced bone formation, and a reduced risk of bone fractures. While previous results have been encouraging, more detailed studies are needed to further develop specific applications. This study demonstrates the effects of Sr 2+ on the osteogenic differentiation of human mesenchymal stromal cells (hMSCs) when introduced as either a dissolved salt, or incorporated into biomimetic calcium phosphate (CaP) coatings. Upon attachment, hMSCs seeded in the presence of higher Sr 2+ concentrations presented with a more elongated shape as compared to the controls without Sr 2+ . Both Sr 2+ as a dissolved salt in the medium, or incorporated into CaP coatings, positively influenced hMSC alkaline phosphatase (ALP) activity in a dose‐dependent manner. At the mRNA level, the expression of osteogenic markers ALP, bone sialoprotein, bone morphogenetic protein 2, osteopontin, and osteoclacin were increased in the presence of Sr 2+ , independent of the delivery method. Overall, this study demonstrates the positive effects of strontium on the osteogenic differentiation of human MSCs, and supports the use of strontium‐incorporated CaPs for bone regeneration applications. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1946–1960, 2016.